Fig. Objective- Dysregulated proliferation of vascular smooth muscle cells (VSMC) plays an essential role in neointimal hyperplasia. A number of growth factors and neurohumoral agents influence smooth muscle growth and differentiation. 1999. By 4 days in vitro, CD36 functions critically in atherogenesis and thrombosis. We have for the first time been able to culture ASM cells from asthmatic patients and to compare their proliferation rate with that of nonasthmatic patients. 123 Restenosis occurs after ≈30% to 40% of the procedures, 14 limiting the utility of … The molecular mechanism underlying SMC proliferation, however, At least four cross-sections per carotid were quantitated. The observation that CDKs 4 and 2 were not activated despite the expression of cyclin D and cyclin E prompted us to examine the expression of Cdc25A and CDK inhibitors. HFD-fed CCL5-/-CCR5-/- mice showed significantly decreased expression of the synthetic phenotype marker osteopontin and the proliferation marker proliferating cell nuclear antigen, and increased expression of the contractile phenotype marker smooth muscle α-actin in the thoracoabdominal aorta vs. wild-type HFD-fed mice. December 14, Excessive proliferation of vascular smooth muscle cells contributes to the etiology of such diseases, including atherosclerosis, restenosis, and pulmonary hypertension. Email to a Friend. Rat carotid arteries were injured using either the gentle injury technique previously described (, Each animal received intraperitoneal injections of tritiated thymidine (specific activity 6.7 Ci/mmol; NEN Life Science Products) at 1, 9, and 17 h prior to being killed with an overdose of sodium pentobarbital (160 mg/kg of body weight; Anthony Products Co., Arcadia, CA) and perfusion-fixed for 5 min with 4% paraformaldehyde in 0.1, Carotid arteries were briefly flushed with Ringer's lactate, excised, stripped of adventitia, and frozen in liquid nitrogen. The feature of PH is intense remodeling of small pulmonary arteries by myofibroblast and smooth muscle cell proliferation, and for familial pulmonary arterial hypertension, the bone morphogenetic protein type II receptor (BMPR-II) mutation in pulmonary artery smooth muscle cells contributes to abnormal growth responses to the transforming growth factor (TGF)-beta/bone morphogenetic protein (BMP) [ 5 Rb regulates cell cycle transit by binding to and inactivating the E2F family of transcription factors. The Human-Specific and Smooth Muscle Cell-Enriched LncRNA SMILR Promotes Proliferation by Regulating Mitotic CENPF mRNA and Drives Cell-Cycle Progression Which Can Be Targeted to Limit Vascular Remodeling Collectively, we demonstrate that SMILR is a critical mediator of vascular SMC proliferation via direct regulation of mitotic progression. Myocardin is a cardiac and smooth muscle-specific coactivator of the ubiquitous SRF transcription factor. 55 year old man with paratesticular mass (Arch Pathol Lab Med 2003;127:E111) 55 year old man with smooth muscle hyperplasia of epididymis (J Surg Case Rep 2011;2011:10) 66 year old man with simultaneous leiomyoma and contralateral smooth muscle hyperplasia of epididymis (Pathologica 2009;101:119) Complex multilocular cystic lesion of rete testis, accompanied by smooth muscle … Liver X receptor agonists suppress vascular smooth muscle cell proliferation and inhibit neointima formation in balloon-injured rat carotid arteries Circ Res. The present study examined the activation of extracellular signal-regulated kinase (ERK) signaling as well as the expression and activity of cell cycle proteins in FGF2-stimulated intimal smooth muscle cells. Please enter a term before submitting your search. DOI: https://doi.org/10.1074/jbc.275.15.11270. The expression of cyclins D and E, however, was not sufficient to induce a high level of proliferation, and we hypothesize that high levels of p15, Received in revised form: Advanced glycation end products (AGEs) have been widely regarded as an important inducing factor in the pathogenesis of diabetic arteriosclerosis, and the proliferation and migration of vascular smooth muscle cells (VSMCs) are also involved in this process. Endothelium-derived nitric oxide (NO) production is both a tonic and an induced regulator of blood vessel tone [1–3]. Smooth muscle cell proliferation was measured by counting the number of labeled nuclei, and the [ 3 H]thymidine index ((labeled nuclei/total nuclei) × 100%) was calculated. 2004 Dec 10;95(12):e110-23. © 2000 ASBMB. The small vessel change is though to be etiologic of the increased peripheral resistance of high blood pressure. Persistent epithelial damage may result in the release of platelet-derived growth factor (PDGF), which stimulates smooth muscle proliferation, followed by fibroblastic proliferation. Smooth muscle proliferation and role of the prostacyclin (IP) receptor in idiopathic pulmonary arterial hypertension. FGF2 stimulation, however, did not lead to phosphorylation of the retinoblastoma protein (Rb), CDK 2 activation, or expression of cyclin A. proliferation of smooth muscle cells, which impairs the contractile phenotype elsewhere. Additionally, the signaling elicited by FGF2 in intimal smooth muscle cells is sufficient to stimulate an increase in the expression of cyclin D, indicating that these cells do enter the cell cycle. SDS was removed from the gel by multiple washes with buffer A (50 m, 150 μg of protein was normalized in 1.0 ml of CDK 2 lysis buffer (50 m, To evaluate the response of medial smooth muscle cells to FGF2, 60 μg of FGF2 was administered intravenously to rats immediately after their carotid artery was subjected to a denuding injury using a nylon filament loop. https://doi.org/10.1016/j.biotechadv.2018.04.006. Copyright © 2021 Elsevier B.V. or its licensors or contributors. Abnormal vascular smooth muscle cell (VSMC) proliferation is involved in restenosis following percutaneous transluminal angioplasty (PTCA) and accelerated arteriosclerosis after cardiac transplantation. Here we report that SRSF1 (serine/arginine-rich splicing factor 1), an essential splicing factor, promotes VSMC proliferation and injury-induced neointima formation. It reported that Long noncoding BRAF-activated noncoding RNA (BANCR) and miR-34c played opposite roles in the regulation of the proliferation of VSMCs, indicating that there might be a potential interaction between them. The article must therefore be hereby marked “, Basic fibroblast growth factor (FGF2) is a potent mitogen for medial smooth muscle cells and is necessary for their proliferation after balloon catheter injury; however, intimal smooth muscle cells do not require FGF2 for their proliferation, and they respond only weakly to exogenous FGF2. MOLECULAR BASIS OF CELL AND DEVELOPMENTAL BIOLOGY, ATTENUATION OF BASIC FIBROBLAST GROWTH FACTOR 2-STIMULATED PROLIFERATION IS ASSOCIATED WITH INCREASED EXPRESSION OF CELL CYCLE INHIBITORS*, Cell Type-specific E2F Activation and Cell Cycle Progression Induced by the Oncogene Product Tax of Human T-cell Leukemia Virus Type I*, Sodium Channel β Subunits Mediate Homophilic Cell Adhesion and Recruit Ankyrin to Points of Cell-Cell Contact*, Quantification of Smooth Muscle Cell Proliferation, Response of Arterial Smooth Muscle Cell to Exogenous FGF2, FGF2 Stimulation of Cytoplasmic Signaling Pathways, Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0), We use cookies to help provide and enhance our service and tailor content and ads. 38 SRF binds to cis DNA regulatory elements called CArG boxes (CC(A/T-rich) 6 GG), which are found in the promoters of muscle-specific genes, as well as serum-inducible genes such as c-fos that regulate proliferation. An. 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